Morphological and molecular characterization of some popular cultivated mushrooms of India

Morphological and molecular characterization of some popular cultivated mushrooms IndiaSanjeev Kumar1*, Anand Sagar2, Shwet Kamal3 Jagriti Rana41. Associate professor, Govt. College Bilaspur.2. Dean Life Sciences, H.P. Univ.. Shimla. 3. Principal Scientist, DMR Solan.4. PhD research Scholar, Department Biosciences, Univ.Shimla.* Corresponding author, E-mail: [email protected] +919418045021 AbstractThe mushroom cultivation is a highly lucrative agribusiness to produce nutritious food earn money in short space time. The present studies were conducted analyze morphological characteristics five strains Agaricus bisporus (NBS-5), Calocybe indica (DMRO-302), Flammulina velutipes (DMRO-253), Pleurotus florida (DMRP-136) Volvariella volvacea (DMRO-484). fruiting bodies procured from ICAR-Directorate Mushroom Research, Solan, HP (India) screened for details. Macroscopic features like shape, size colour stipe pileus recorded. Microscopic details worked out by cutting free hand sections material observed under microscope scanning electron microscope. Molecular identification was done through internal transcribed spacer (ITS) regions ribosomal DNA sequencing. nucleotide sequences each sample analyzed Basic local Alignment Search Tool (BLAST) search program US National Centre Biotechnology Information (NCBI) identification. obtained this study submitted the Gen Bank database at NCBI with accession numbers MW718994.1 (A. bisporus), MW714627.1 (C. indica), MW719474.1 (F. velutipes), MW719254.1 (P. florida) MW720603.1 (V. volvacea).Keywords: Mushroom, identification, ITS, rRNA, Nucleotide sequencing,